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2 edition of Principles and performance of the rubella hemagglutination inhibition test found in the catalog.

Principles and performance of the rubella hemagglutination inhibition test

Edwin H. Lennette

Principles and performance of the rubella hemagglutination inhibition test

by Edwin H. Lennette

  • 313 Want to read
  • 17 Currently reading

Published by American Society of Clinical Pathologists in Chicago, Ill .
Written in English

    Subjects:
  • Rubella -- Immunological aspects.

  • Edition Notes

    Bibliography: leaves 29-31.

    Statement[by] Edwin H. Lennette [and] Nathalie J. Schmidt.
    ContributionsSchmidt, Nathalie J., joint author.
    Classifications
    LC ClassificationsRC182.R8 L44
    The Physical Object
    Pagination33 l.
    Number of Pages33
    ID Numbers
    Open LibraryOL5438497M
    LC Control Number73087427

    Serum specimens from 80 patients with proven rheumatoid arthritis (R.A.) were tested for the presence of rubella haemagglutination-inhibition antibody, and the results were compared with similar data on an equal number of well, non-arthritic controls and a group of 62 individuals with other forms of by: Occurrence. Rubella occurs worldwide. See information about clinical case definition, clinical classification and epidemiologic classification of rubella and congenital rubella syndrome.. Reservoir. Rubella is a human disease. There is no known animal reservoir. Although infants with CRS may shed rubella virus for an extended period, a true carrier state has not been described.

    THE isolation of rubella virus1, 2 in opened a period of remarkable progress in studies of the virus,3 the disease4 and, recently, experimental vaccines for preventing the disease.5, 6 Compa Cited by: Principles and performance of the rubella hemagglutination inhibition test by: Lennette, Edwin H., Published: () Epidemiology of rubella and its prevention: with special reference to the findings in Hokkaido, Japan / by: Ishii, Keizo. Published: ().

    -Hemagglutination inhibition, complement fixation, radial hemolysis, latex agglutination, ELISA EIA theory -If the test specimen does not contain IgG antibody --Antigen conjugate will not bind to the well surface and will be removed by washing. Hemagglutination. Treated animal RBC is used as a carrier of antigen; Passive hemagglutination: Ag that are being bound by Ab are not the Ag of RBC but are passively bound Ag. Examples. Microhemagglutination test for Syphilis (MHA-TP) Hemagglutination treponemal test for Syphilis (HATTS) Passive hemagglutination tests for antibody to extracellular antigen of Streptococci.


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Principles and performance of the rubella hemagglutination inhibition test by Edwin H. Lennette Download PDF EPUB FB2

Principles and performance of > Staff View; Cite this; Text this; Export Record. Export to EndNoteWeb; Export to EndNote; Export to MARC; Export to RDF; Principles and performance of the rubella hemagglutination inhibition test.

Main Author: Lennette, Edwin H., a Principles and performance of the rubella hemagglutination. Principles and performance of the rubella hemagglutination inhibition test by Edwin H.

Lennette Published by American Society of Clinical Pathologists in Chicago, : Rubella: first annual symposium of the Eastern Pennsylvania Branch, American Society for Microbiology.

Published: () Epidemiology of rubella and its prevention: with special reference to the findings in Hokkaido, Japan / by: Ishii. (NSI) ofrubella virus hemagglutination (HA) (5, 8). It might appear, therefore, that for the rubella hemagglutination-inhibition (HI) test, bacterial contamination ofthis sort would be advantageous rather than the opposite.

How-ever, partial hydrolysis of the phospholipid moieties bythis enzymehasbeen found to in-terfere with the formation of Cited by: 1. This corrects the article "Rubella—Technical Problems in the Performance of Hemagglutination-Inhibition Tests" in volume on page Full text Get a printable copy (PDF file) of the complete article (81K), or click on a page image below to browse page by page.

RUBELLA HEMAGGLUTINATION INHIBITION tion resulted in anincrease ofHItiter from64 to 4, (Table 2). BecauseoftheheatstabilityofPL-C,atestof whether the complete inactivation ofthe lipo-proteins by PL-C was due to the Pb-C or to some other enzymes contaminating the PL-C preparation wasdonebyheating a solution of the enzyme ( unit/ml) for 15 min at 70°C.

This heated PL-C Cited by: 1. The hemagglutination inhibition test for rubella: a comparison of its sensitivity to that of neutralization, complement fixation and fluorescent antibody tests for diagnosis of infection and determination of immunity : Edwin H. Lennette, Nathalie J. Schmidt. The hemagglutination inhibition test for rubella: a comparison of its sensitivity to that of neutralization, complement fixation and fluorescent antibody tests for diagnosis of infection and determination of immunity by: 7.

Ascreen test for rubella haemagglutination inhibition antibodies W.R. THOMASANDWENDYJ. KEMPSELL Fromthe GroupLaboratories, MaydayHospital, Croydon,and hemagglutination inhibition test. New Engi. Med.,Arapid staining technique for principles and specificity ofGomori's me£hod.

The procedure can becompletedin five Cited by: 4. Hemagglutination Inhibition Test (HAI): Principle, procedure, result and interpretations Decem Acharya Tankeshwar Lab Diagnosis of Viral Disease, Virology 7 The nucleic acids of various viruses encode surface proteins (lutinin (HA) of influenza virus) that agglutinate red blood cells (RBC) of a variety of species.

Rubella—Technical Problems in the Performance of Hemagglutination-Inhibition (HI) Tests. By Edwin H. Lennette and Nathalie J.

Schmidt. Abstract. The rubella hemagglutination inhibition (hi) test is the most sensitive serologic method available for diagnosis of infection and for determination of immunity status.

At present, however, it is not Author: Edwin H. Lennette and Nathalie J. Schmidt. Evaluation ofa Rubella HemagglutinationInhibition Test System JOHN A.

SMITH* AND ALVIN C. CUMMINS Division ofMicrobiology, MountSinai Hospital, Toronto, Ontario M5G1X5Canada Received for publication 14 August Two systems for measurement of rubella hemagglutination inhibition anti-bodies were compared.

One had chick erythrocytes as Cited by: 2. The authors have studied the haemagglutination-inhibition test in comparison with other methods for the diagnosis of rubella. BHK cells were infected with rubella virus and incubated in a maintenance medium containing 2% of kaolin-treated fetal calf serum.

The culture fluids were collected after 6 days and treated with Tween 80 and ether; they contained haemagglutinating antigen in titres of Cited by: Principles and performance of the rubella hemagglutination inhibition test. Chicago, Ill.: American Society of Clinical Pathologists.

MLA Citation. Lennette, Edwin H., and Nathalie J. Schmidt. Principles and Performance of the Rubella Hemagglutination Inhibition Test. Chicago, Ill.: American Society of Clinical Pathologists,   A method using phospholipase C (PL-C) for removing nonspecific inhibitors (NSI) of rubella virus hemagglutinin is described.

PL-C was found to hydrolyze NSI without altering the hemagglutination inhibition (HI) activity of the specific antibody and could be used to remove NSI in the rubella HI test by using formalinized erythrocytes, which resisted the enzymatic action; fresh Cited by: 1.

solution are satisfactory for rubella HItests for as long as 2 weeks. Effect of composition and pH of diluents on rubella antigen and antibody titers.

Various dilu-ents have been described for rubella HI test systems. Some ofthese (5, 6, 10; H. Liebhaber, personal communication) give a final pH of in the test, which has been reported to be theCited by: The rubella hemagglutination-inhibition (HI) test (6) is widely used for determination ofsero-immunity andfor the diagnosis ofrecent rubella infection.

By and large, however, the test has been used on serum samples only. Efforts to extend this test to whole blood by absorbing the blood on filter paper discs, eluting with buffers Author: Jiban D. Gupta, Vincent J. Peterson, John D. Harley. The latex agglutination card test (Rubascan) for the detection of rubella antibody was compared with the standard hemagglutination inhibition and enzyme-linked immunosorbent assay tests.

There was complete agreement with sera which had hemagglutination inhibition titers of ≥ Sera with low levels of antibody which were positive in the enzyme-linked immunosorbent assay, however, gave Cited by: 8.

The test result is read visually after an incubation period of 15–30 minutes. When compared with a hemagglutination inhibition assay, the Rubaquick assay results obtained from 1, sera were greater than 99% specific, sensitive, and by: 5.

The modification of the standard rubella haemagglutination-inhibition (HAI) test using trypsin-treated human group O erythrocytes instead of chick erythrocytes was evaluated.

In a comparative study we found that, of samples tested by both methods, the titres of (%) sera were in close agreement within an acceptable twofold difference. Trypsin-treated human group O erythrocytes usually provided titres Cited by: 4. influencingthe reliability ofthetest are discussed.

Thehemagglutination-inhibition (HI) testfor rubella has foundwideapplication in the diag-nosis of rubella infections and in the determi-nation of immunity status (3, 6, 8, 10, 19); for routine use, this test has largely replaced the more cumbersome and expensive neutraliza-tionandfluorescent.A procedural guide to the performance of rubella hemagglutination-inhibition tests.

Procedural guide to the performance of rubella hemagglutination-inhibition tests. Atlanta, Ga.: U.S. Dept. of Health, Education, and Welfare, Public Health Service, Center for Disease Control, Bureau of Laboratories, [] http:\/\/Lennette EH, Schmidt NJ, Magoffin RL.

The hemagglutination inhibition test for rubella: a comparison of its sensitivity to that of neutralization, complement fixation and fluorescent antibody tests for diagnosis of infection and determination of immunity status. J Immunol. Oct; 99 (4)– Mann JJ, Rossen RD, Lehrich JR, Kasel JA.